The Church of the Holy Trinity is a Greek Orthodox church in Brighton, part of the English city of Brighton and Hove.

Built in 1838 in Carlton Hill, it was an Anglican church for most of its life: dedicated to St John the Evangelist. After some uncertainty about its future, it was sold to Brighton’s Greek Orthodox community in 1985 and has been used as their permanent place of worship since then. Reflecting its architectural and historical importance, it has been listed at Grade II since 1971.

Since the Greek Orthodox community acquired the building, some interior alterations have been made, including the installation of a new altar screen. It has been licensed for worship in accordance with the Places of Worship Registration Act 1855 and has the registration number 79056.

Built in brick (in a Flemish bond pattern) with some stone dressings, the church has a stuccoed southern frontage, facing Carlton Hill. A deep central recess is flanked by two prominent wings with entrance door and large stone pilasters, above which is a pediment with an embedded clock. The large crucifix above the entrance is a recent addition. The Georgian-style front elevation, was improved in 1957 by L.A. Mackintosh, whose personal monogram is on the wall above the left entrance door. The church was listed at Grade II by English Heritage on 20 August 1971.

The church was badly damaged by fire in July 2010.

In the early afternoon of Sunday 4 July, fire spread from the ground floor through to parts of the roof. There were no casualties. Although there was no structural damage,the entire interior was gutted causing £500,000 worth of damage.

In early 2018, after more than £80 000 of repair work was carried out, the front facade of the church was renovated fully. The iconic clock was also replaced and the church has been restored to its former glory.